il 1β (R&D Systems)
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il 1β
Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+cxcl8/Recombinant+Human+IL-8%2FCXCL8+Protein/pmc13107899-34-10-22
Average 95 stars, based on 133 article reviews
Il 1β, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+cxcl8/Recombinant+Human+IL-8%2FCXCL8+Protein/pmc13107899-34-10-22
Average 95 stars, based on 133 article reviews
il 1β - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling"
Article Title: IL-1β modulates inflammatory response of human bone marrow-derived MSCs and neutrophil recruitment in vitro via NF-kB-associated signaling
Journal: Stem Cell Research & Therapy
doi: 10.1186/s13287-026-05029-x
Figure Legend Snippet: IL-1β stimulation changes the transcriptomic profile of BM-hMSCs. Schematic of experimental design A . BM-hMSCs were exposed to IL-1 β (3 replicates, 1 donor) or left unstimulated (3 replicates, 1 donor) for one hour, followed by bulk RNA sequencing. PCA plot was used to show the variance between the unstimulated control (green) and IL-1 β exposed (purple) samples B . Heatmap displaying the Z-score of the top 100 varying genes across all samples. Red color indicates higher expression of the genes and blue color indicates decreased expression C . Volcano plot of differentially expressed genes, showing their log2 fold change (X-axis) and -log10 adjusted p-values (Y-axis) D . IL-1 β Interleukin-1β, BM-hMSCs, Bone marrow derived human mesenchymal cells, PCA Principal component analysis, FC Fold change, NS not significant. Figure 1A was created using Biorender.com
Techniques Used: RNA Sequencing, Control, Expressing, Derivative Assay
Figure Legend Snippet: IL-1β stimulation increases expression of neutrophil recruitment and NF-kB signaling genes by BM-hMSCs. Heatmap clustering of the Z-score of the top 20 differentially expressed genes between unstimulated control BM-hMSCs (3 replicates, 1 donor, green) and IL-1 β stimulated BM-hMSCs (3 replicates, 1 donor, purple). Red color indicates higher expression of the genes and blue color indicates decreased expression A . Protein expression of CXCL1 (10 replicates, 4 donors), CCL2 (4 replicates, 2 donors), CXCL5 (4 replicates, 2 donors), and CXCL8/IL-8 (7 replicates, 3 donors) in BM-hMSCs secretome measured by ELLA or ELISA. Data are presented as median, and statistical analysis was performed using unpaired t-test with Welch’s correction B . Bar plot of the top 20 significant Gene Ontology (GO) biological processes representing IL- 1β induced upregulated differentially expressed genes C IL-1 β, Interleukin-1β; BM-hMSCs, Bone marrow derived human mesenchymal cells; CCL2, chemokine (C–C motif) ligand 2; CXCL1, chemokine (C-X-C motif) ligand 1; CXCL2, chemokine (C-X-C motif) ligand 1; CXCL8, chemokine (C-X-C motif) ligand 8; ****, p < 0,0001
Techniques Used: Expressing, Control, Enzyme-linked Immunosorbent Assay, Derivative Assay
Figure Legend Snippet: IL-1β stimulated BM-hMSCs increased neutrophil recruitment partly via the NF-kB signaling pathway. Schematic of experimental design of the neutrophil migration assay. Illustration was created with BioRender.com A . Number of neutrophils that migrated through the transwell membrane from the top well to the bottom towards control conditioned medium, conditioned medium from IL-1β stimulated BM-hMSCs, and conditioned medium from IL-1β with the NF-kB inhibitor stimulated BM-hMSCs. The experiment was performed using neutrophils from four different donors (each donor represent one data point) and the experiments were performed on three different days. B . Protein expression of CXCL1 was measured in conditioned medium from unstimulated BM-hMSCs (4 replicates, 1 donor, green), IL-1β stimulated BM-hMSCs (4 replicates, 1 donor, purple), and ILβ stimulated BM-hMSCs with 10 μM BAY 11–7082 (4 replicates, 1 donor, grey) C . Phospho-p65 levels were quantified and normalized to total p65. Data are expressed relative to control, which was set to 1 D . IL-1β Interleukin-1β BM-hMSCs Bone marrow-derived human mesenchymal cells, Ctrl control, CXCL1 chemokine (C-X-C motif) ligand 1, ns not significant; * p < 0,05; ****, p < 0,0001
Techniques Used: Migration, Membrane, Control, Expressing, Derivative Assay
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![A. In B16F10 pulmonary melanoma tissue, mRNA expression of MCP1 and IL-10 increased following treatment by Pembrolizumab or rhARSB, and the increase was enhanced by their combination. In contrast, IL-17α expression was increased by Pembrolizumab, but not by rhARSB. B. KC, the mouse analog of <t>IL-8,</t> and IL-6 were reduced by both rhARSB and Pembrolizumab and further reduced by their combination. In contrast, TNFα was increased by Pembrolizumab and reduced by rhARSB, with overall reduction by their combination. C. FGF2, VEGF, and EGF were reduced by ARSB, but not by Pembrolizumab, and the combination of ARSB and Pembrolizumab did not lead to additional decline. [ARSB=arylsulfatase B=N-acetylgalactosamine-4-sulfatase; EGF=epidermal growth factor; bFGF=basic fibroblast growth factor; IL=interleukin; KC=CXCL1=keratinocyte-derived cytokine; rh=recombinant human; MCP1=CCL2= monocyte chemoattractant protein-1; VEGF=vascular endothelial growth factor]](https://med-rxiv-images-cdn.bioz.com/dois_ending_with_95/10__64898_slash_2026__01__02__25343295/10__64898_slash_2026__01__02__25343295___F4.large.jpg)
![A. Although KC expression was overall reduced by rhARSB in the mouse pulmonary metastases ( Fig.4B ), IL-8 in the spent media of A375 cells following ARSB increased (p=2.5×10 -5 ) and declined when ARSB was silenced (p=2.5×10 -5 ). In contrast, IL-8 in the cell extract declined following rhARSB declined following rhARSB (p=1.1×10 -7 ). B. PMN invasion to the A375 cells was increased by rhARSB (p=0.0041), reduced by ARSB siRNA (p=0.0026), reduced by IL-8 silencing (p=0.00027), and increased by addition of rhIL-8 (p=6.1×10 -5 ). These effects are attributable to increased IL-8 in the spent media following rhARSB. C. PMN migration from the filter on top to the bottom well was increased by IL-8 in the bottom well, but unaffected by Pembrolizumab. D. When IL-8 was silenced in the A375 cells, the effect of rhARSB + PBMC(Ac) and of Pembro + PBMC(Ac) and their combination [rhARSB+Pembro+PBMC(Ac)] on cleaved caspase-3 was reduced, consistent with an effect of IL-8 on PBMC(Ac)-mediated apoptosis. E. When PMN were added to the combination of rhARSB+Pembrolizumab+PBMC, the cleaved caspase-3 was further increased in the A375 cells (p=0.03). [Ac=activated, consi=control siRNA; IL=interleukin; rh=recombinant human; Pembro=Pembrolizumab; PBMC=peripheral blood mononuclear cells; PMN=polymorphonuclear leukocytes; si=siRNA]](https://med-rxiv-images-cdn.bioz.com/dois_ending_with_95/10__64898_slash_2026__01__02__25343295/10__64898_slash_2026__01__02__25343295___F5.large.jpg)